Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Quantity 50 µg
Quantitation Method Please see our FAQ
Biopsy Source Lung
Cell Type Fibroblast
Tissue Type Lung
Transformant Untransformed
Race Black
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN 46,XX.arr[hg19] 15q11.1q11.2(20646562-22383189)x1
Species Homo sapiens
Common Name Human
Remarks Lung biopsy; same patient as GM06111; 46,XX; 4% of cells show random chromosome loss.
Passage Frozen 3
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Remark Lung biopsy; same patient as GM06111; 46,XX; 4% of cells show random chromosome loss.
Sassi-Gaha S, Loughlin DT, Kappler F, Schwartz ML, Su B, Tobia AM, Artlett CM, Two dicarbonyl compounds, 3-deoxyglucosone and methylglyoxal, differentially modulate dermal fibroblasts Matrix biology : journal of the International Society for Matrix Biology29:127-34 2009
PubMed ID: 19800404
 
Mansur NR, Meyer-Siegler K, Wurzer JC, Sirover MA, Cell cycle regulation of the glyceraldehyde-3-phosphate dehydrogenase/uracil DNA glycosylase gene in normal human cells. Nucleic Acids Res21:993-8 1993
PubMed ID: 8451199
No data is available
Passage Frozen 3
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA