Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
Quantity 50 µg
Quantitation Method Please see our FAQ
Biopsy Source Unspecified
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Black
Family Member 1
Relation to Proband proband
Confirmation Karyotypic analysis and Case history
ISCN 46,XX.arr 7q11.23(73728758-73964249)x1,8p23.1(7284737-7791790)x1
Species Homo sapiens
Common Name Human
Remarks Skin biopsy; same patient as GM06112; 46,XX; 6% of cells show random chromosome loss
PDL at Freeze 7.05
Passage Frozen 7
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Remark Skin biopsy; same patient as GM06112; 46,XX; 6% of cells show random chromosome loss
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013
PubMed ID: 23665875
 
Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994
PubMed ID: 8106562
 
Ethier MF, Hickler RB, Dobson JG Jr, Aging increases adenosine and inosine release by human fibroblast cultures. Mech Ageing Dev50:159-68 1989
PubMed ID: 2601414
No data is available
Passage Frozen 7
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified