Coriell Institute
Repository NIGMS Human Genetic Cell Repository
Subcollection Chromosome Abnormalities
Cell Type Fibroblast
Transformant Untransformed
Relation to Proband proband
Confirmation Karyotypic analysis after cell line submission to CCR
ISCN 47,XX,+8[75]/46,XX,+8,dic(14;21)(14qter>14p13::21p13> 21qter)[25]
Species Homo sapiens
Common Name Human
Remarks Additional t(14;21) present in only 25% of cells at passage 6; passage 4 at IMR
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
Cytogenetics Chromosome 8: ANEUPLOID Aneuploid Segment (+)8pter>8qter
Chromosome 8: ANEUPLOID Trisomic Segment 8pter>8qter
Remark Additional t(14;21) present in only 25% of cells at passage 6; passage 4 at IMR
Treff NR, Tao X, Ferry KM, Su J, Taylor D, Scott RT, Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy screening Fertility and sterility97:819-824.e2 2011
PubMed ID: 22342859
Treff NR, Su J, Tao X, Levy B, Scott RT, Accurate single cell 24 chromosome aneuploidy screening using whole genome amplification and single nucleotide polymorphism microarrays Fertility and sterility94:2017-21 2009
PubMed ID: 20188357
No data is available
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA

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