Repository NIGMS Human Genetic Cell Repository
Subcollection Chromosome Abnormalities
dbGaP
Quantity 50 µg
Quantitation Method Please see our FAQ
Cell Type Fibroblast
Transformant Untransformed
Race Caucasian
Relation to Proband proband
Confirmation Karyotypic analysis after cell line submission to CCR
ISCN 47,XY,+8[29]/46,XY[21].arr 8p23.3 q24.3(36385-146268947)x3,17q25.1(69343532-70188895)x3
Species Homo sapiens
Common Name Human
Remarks In fibroblasts 58% have +8; mental retardation
Passage Frozen 7
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Cytogenetics Chromosome 8: ANEUPLOID Aneuploid Segment (+)8pter>8qter
Chromosome 8: ANEUPLOID Trisomic Segment 8pter>8qter
Remark In fibroblasts 58% have +8; mental retardation
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013
PubMed ID: 23665875
 
de la Chapelle A, Icen A, Aula P, Leisti J, Turleau C, de Grouchy J, Mapping of the gene for glutathione reductase on chromosome 8. Ann Genet19:253-6 1976
PubMed ID: 1087855
No data is available
Passage Frozen 7
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA