Coriell Institute
Repository NIGMS Human Genetic Cell Repository
Subcollection Chromosome Abnormalities
dbGaP
Quantity 50 µg
Quantitation Method Please see our FAQ
Cell Type Fibroblast
Transformant Untransformed
Relation to Proband proband
Confirmation Karyotypic analysis after cell line submission to CCR
ISCN 47,XY,+8[81]/46,XY[19].arr(8)x3
Species Homo sapiens
Common Name Human
Remarks Mosaic; in fibroblasts, 81% are 47,XY,+8; in lymphocytes, 83% are 47,XY,+8
Passage Frozen 3
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 
Cytogenetics Chromosome 8: ANEUPLOID Aneuploid Segment (+)8pter>8qter
Chromosome 8: ANEUPLOID Trisomic Segment 8pter>8qter
Remark Mosaic; in fibroblasts, 81% are 47,XY,+8; in lymphocytes, 83% are 47,XY,+8
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013
PubMed ID: 23665875
 
Su TS, Nussbaum RL, Airhart S, Ledbetter DH, Mohandas T, O'Brien WE, Beaudet AL, Human chromosomal assignments for 14 argininosuccinate synthetase pseudogenes: cloned DNAs as reagents for cytogenetic analysis. Am J Hum Genet36:954-64 1984
PubMed ID: 6093508
 
Chen YT, Worthy TE, Krooth RS, Evidence for a striking increase in acetylcholinesterase activity in cultured human fibroblasts which are trisomic for chromosome two. Somatic Cell Genet4:265-98 1978
PubMed ID: 694721
No data is available
Passage Frozen 3
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA

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