Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Protocols Protocol PDF
Biopsy Source Skin
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Sendai)
Race Caucasian
Ethnicity CANADIAN
Family Member 57
Family History Y
ISCN 46,XX[25].arr(1-22,X)x2
Species Homo sapiens
Common Name Human
Remarks The donor, now deceased, was affected with Alzheimer's disease. Onset of the disease occured at age 45. This diagnosis was updated by the submitter on 3/2009. The skin biopsy was taken ante-mortem from the forearm. A lymphoblast culture from same donor is AG07767.
Passage Frozen 16
 
Induced Pluripotent Stem Cell The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 
Remark The donor, now deceased, was affected with Alzheimer's disease. Onset of the disease occured at age 45. This diagnosis was updated by the submitter on 3/2009. The skin biopsy was taken ante-mortem from the forearm. A lymphoblast culture from same donor is AG07767.
No data is available
No data is available
Passage Frozen 16
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 20% Knock-out Serum Replacement Not inactivated
Substrate Gelatin + Feeder Layer
Supplement Basic Fibroblast Growth Factor 10 ng/ml