Coriell Institute
Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Quantity 10 µg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Donor is clinically affected with Alzheimer's disease. Culture was initiated on 9/3/81 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. The APOE genotype of the donor subject is E2/E3. A fibroblast cell culture from this same donor is AG21158.
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin confirmed by LINE assay
 
Gene APOE
Chromosomal Location 19q13.2
Allelic Variant 1 107741.0001; APOE2 ISOFORMS
Identified Mutation ARG158CYS; The 3 major isoforms of human apolipoprotein E (apoE2, -E3, and -E4), as identified by isoelectric focusing, are coded for by 3 alleles (epsilon 2, 3, and 4). The E2 (107741.0001), E3 (107741.0015), and E4 (107741.0016) isoforms differ in amino acid sequence at 2 sites, residue 112 (called site A) and residue 158 (called site B). At sites A/B, apoE2, -E3, and -E4 contain cysteine/cysteine, cysteine/arginine, and arginine/arginine, respectively [Weisgraber et al. J. Biol. Chem. 256: 9077-9083 (1981) and Rall et al. Proc. Nat. Acad. Sci. 79: 4696-4700 (1982)].
 
Gene APOE
Chromosomal Location 19q13.2
Allelic Variant 2 107741.0015; APOE3 ISOFORM
Identified Mutation CYS112 AND ARG158; Weisgraber et al. [J. Biol. Chem. 256: 9077-9083 (1981)] and Rall et al. [Proc. Nat. Acad. Sci. 79: 4696-4700 (1982)] identified one of the 3 major apolipoprotein E isoforms, apolipoprotein E3. The variant has Cys112 and Arg158. This is the most common variant, with frequencies of 40% to 90% in various populations.
Remark Donor is clinically affected with Alzheimer's disease. Culture was initiated on 9/3/81 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. The APOE genotype of the donor subject is E2/E3. A fibroblast cell culture from this same donor is AG21158.
No data is available
No data is available
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium

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