Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Quantity 10 µg
Quantitation Method Please see our FAQ
Biopsy Source Unspecified
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The donor is clinically affected with Alzheimer's disease. The biopsy was taken ante-mortem. The culture was initiated on 9/3/81 using explants of minced skin tissue. The cell morphology is fibroblast-like. The APOE genotype of the donor subject is E2/E3. A lymphoblast cell culture from this same donor is AG21159.
PDL at Freeze 5.42
Passage Frozen 1
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by LINE assay
 
Gene APOE
Chromosomal Location 19q13.2
Allelic Variant 1 107741.0001; APOE2 ISOFORMS
Identified Mutation ARG158CYS; The 3 major isoforms of human apolipoprotein E (apoE2, -E3, and -E4), as identified by isoelectric focusing, are coded for by 3 alleles (epsilon 2, 3, and 4). The E2 (107741.0001), E3 (107741.0015), and E4 (107741.0016) isoforms differ in amino acid sequence at 2 sites, residue 112 (called site A) and residue 158 (called site B). At sites A/B, apoE2, -E3, and -E4 contain cysteine/cysteine, cysteine/arginine, and arginine/arginine, respectively [Weisgraber et al. J. Biol. Chem. 256: 9077-9083 (1981) and Rall et al. Proc. Nat. Acad. Sci. 79: 4696-4700 (1982)].
 
Gene APOE
Chromosomal Location 19q13.2
Allelic Variant 2 107741.0015; APOE3 ISOFORM
Identified Mutation CYS112 AND ARG158; Weisgraber et al. [J. Biol. Chem. 256: 9077-9083 (1981)] and Rall et al. [Proc. Nat. Acad. Sci. 79: 4696-4700 (1982)] identified one of the 3 major apolipoprotein E isoforms, apolipoprotein E3. The variant has Cys112 and Arg158. This is the most common variant, with frequencies of 40% to 90% in various populations.
Remark The donor is clinically affected with Alzheimer's disease. The biopsy was taken ante-mortem. The culture was initiated on 9/3/81 using explants of minced skin tissue. The cell morphology is fibroblast-like. The APOE genotype of the donor subject is E2/E3. A lymphoblast cell culture from this same donor is AG21159.
Meyer K, Feldman HM, Lu T, Drake D, Lim ET, Ling KH, Bishop NA, Pan Y, Seo J, Lin YT, Su SC, Church GM, Tsai LH, Yankner BA, REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease Cell reports26:1112-1127.e9 2018
PubMed ID: 30699343
 
Scudiero DA, Polinsky RJ, Brumback RA, Tarone RE, Nee LE, Robbins JH, Alzheimer disease fibroblasts are hypersensitive to the lethal effects of a DNA-damaging chemical. Mutat Res159:125-31 1986
PubMed ID: 3941662
No data is available
Passage Frozen 1
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 3%
Medium Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM
Serum 15% fetal bovine serum Not inactivated