Coriell Institute
Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Caucasian
Ethnicity ITALIAN
Family Member 5
Relation to Proband father
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The donor is an unaffected spouse of an Alzheimer's disease affected family member. The culture was initiated on 1/30/90 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. Donor's children are AG10825B, AG10822, AG10823A, and AG10821.
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark The donor is an unaffected spouse of an Alzheimer's disease affected family member. The culture was initiated on 1/30/90 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical. Donor's children are AG10825B, AG10822, AG10823A, and AG10821.
No data is available
No data is available
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium

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