Repository NIA Aging Cell Culture Repository
Subcollection Animals
Biopsy Source Trachealis muscle
Cell Type Smooth muscle
Tissue Type Muscle
Transformant Untransformed
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Oryctolagus cuniculus
Common Name rabbit
Remarks Clone LTSMC-3G. This culture is a clonal derivative of line LTSMC-3, AG10187, from a 6 month old male New Zealand White rabbit, initiated on 7/14/88 using cells released by protease/collagenase digestion. Cell morphology is epithelial-like. In vitro lifespan studies on this culture were halted at PDL 129. This culture may be immortal. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay).
PDL at Senescence 129
PDL at Freeze 25
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark Clone LTSMC-3G. This culture is a clonal derivative of line LTSMC-3, AG10187, from a 6 month old male New Zealand White rabbit, initiated on 7/14/88 using cells released by protease/collagenase digestion. Cell morphology is epithelial-like. In vitro lifespan studies on this culture were halted at PDL 129. This culture may be immortal. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay).
No data is available
No data is available
PDL at Senescence 129
Cumulative PDL at Freeze 25
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Ham's F12 with 2mM L-glutamine or equivalent
Serum 20% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA