Coriell Institute
Repository NIA Aging Cell Culture Repository
Subcollection Animals
Biopsy Source Trachealis muscle
Cell Type Smooth muscle
Tissue Type Muscle
Transformant Untransformed
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Oryctolagus cuniculus
Common Name rabbit
Remarks Line LTSMC-3. A surgical specimen dissected free from the trachea of a New Zealand White rabbit was removed 5 minutes post-mortem on 7/14/88. Cells were released by protease/collagenase digestion for the primary culture. Cell morphology is epithelial-like. In vitro lifespan studies on this culture were halted at PDL 155. This culture may be immortal. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay).
PDL at Senescence 155
PDL at Freeze 10
Passage Frozen 3
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Genbank COX1
Remark Line LTSMC-3. A surgical specimen dissected free from the trachea of a New Zealand White rabbit was removed 5 minutes post-mortem on 7/14/88. Cells were released by protease/collagenase digestion for the primary culture. Cell morphology is epithelial-like. In vitro lifespan studies on this culture were halted at PDL 155. This culture may be immortal. The culture tested positive for alpha-smooth muscle actin (immunofluorescence assay).
Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999
PubMed ID: 10559316
No data is available
PDL at Senescence 155
Cumulative PDL at Freeze 10
Passage Frozen 3
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 with 2mM L-glutamine or equivalent
Serum 20% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA

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