Repository NIA Aging Cell Culture Repository
Subcollection German Alzheimer Disease
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Caucasian
Ethnicity GERMAN
Family Member 26
Relation to Proband VI-06
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The donor is 50% at risk for Alzheimer's disease. The culture was initiated on 10/21/86 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical.
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark The donor is 50% at risk for Alzheimer's disease. The culture was initiated on 10/21/86 by transformation of lymphocytes with Epstein Barr virus. The cells grow in suspension and their morphology is spherical.
Frommelt P, Schnabel R, Kuhne W, Nee LE, Polinsky RJ, Familial Alzheimer disease: a large, multigeneration German kindred. Alzheimer Dis Assoc Disord5:36-43 1991
PubMed ID: 2025423
 
St George-Hyslop PH, Tanzi RE, Polinsky RJ, Haines JL, Nee L, Watkins PC, Myers RH, Feldman RG, Pollen D, Drachman D, et al, The genetic defect causing familial Alzheimer's disease maps on chromosome 21. Science235:885-90 1987
PubMed ID: 2880399
No data is available
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium