AG08452
Fibroblast from Skin, Arm
Description:
MACACA
ORDER: PRIMATES
NIA AGING CELL REPOSITORY DNA PANEL - PRIMATE PANEL: PHYLOGENETIC
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Repository
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NIA Aging Cell Culture Repository
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Permit Requirements
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CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
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| Subcollection |
Animals |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX
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Species
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Macaca nemestrina
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Common Name
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pigtailed macaque
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Remarks
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| PDL at Senescence |
104 |
| PDL at Freeze |
4 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Genbank |
Apolipoprotein B |
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| Genbank |
Lipoprotein Lp(a) |
| Remark |
The skin biopsy was taken post-mortem on 10/21/85 from the inner aspect of the upper arm of a fetus of 131 days gestational age. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblastlike. The karyotype is 42,XX; normal diploid female. A matching lung fibroblast culture from this monkey is AG08453. |
| Kelley JL, Swanson WJ, Dietary change and adaptive evolution of enamelin in humans and among primates Genetics178:1595-603 2008 |
| PubMed ID: 18245370 |
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| Hahn Y, Jeong S, Lee B, Inactivation of MOXD2 and S100A15A by Exon Deletion during Human Evolution Molecular biology and evolution24:2203-12 2007 |
| PubMed ID: 17642472 |
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| Steiper ME, Parikh SJ, Zichello JM, Phylogenetic analysis of the promoter region of the CD40L gene in primates and other mammals Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases8:406-13 2006 |
| PubMed ID: 17275421 |
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| Ireland J, Carlton VE, Falkowski M, Moorhead M, Tran K, Useche F, Hardenbol P, Erbilgin A, Fitzgerald R, Willis TD, Faham M, Large-scale characterization of public database SNPs causing non-synonymous changes in three ethnic groups. Hum Genet119(1-2):75-83 2006 |
| PubMed ID: 16391945 |
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| Xing J, Wang H, Han K, Ray DA, Huang CH, Chemnick LG, Stewart CB, Disotell TR, Ryder OA, Batzer MA, A mobile element based phylogeny of Old World monkeys Molecular phylogenetics and evolution37:872-80 2005 |
| PubMed ID: 15936216 |
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| Clark NL, Swanson WJ, Pervasive adaptive evolution in primate seminal proteins PLoS genetics1:e35 2005 |
| PubMed ID: 16170411 |
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| Salem AH, Ray DA, Hedges DJ, Jurka J, Batzer MA, Analysis of the human Alu Ye lineage. BMC Evol Biol5(1):18 2005 |
| PubMed ID: 15725352 |
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| Rockman MV, Hahn MW, Soranzo N, Loisel DA, Goldstein DB, Wray GA, Positive selection on MMP3 regulation has shaped heart disease risk Current biology : CB14:1531-9 2004 |
| PubMed ID: 15341739 |
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| Stedman HH, Kozyak BW, Nelson A, Thesier DM, Su LT, Low DW, Bridges CR, Shrager JB, Minugh-Purvis N, Mitchell MA, Myosin gene mutation correlates with anatomical changes in the human lineage. Nature428(6981):415-8 2004 |
| PubMed ID: 15042088 |
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| Boffelli D, McAuliffe J, Ovcharenko D, Lewis KD, Ovcharenko I, Pachter L, Rubin EM, Phylogenetic shadowing of primate sequences to find functional regions of the human genome. Science299(5611):1391-4 2003 |
| PubMed ID: 12610304 |
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| Salem AH, Ray DA, Xing J, Callinan PA, Myers JS, Hedges DJ, Garber RK, Witherspoon DJ, Jorde LB, Batzer MA, Alu elements and hominid phylogenetics. Proc Natl Acad Sci U S A100(22):12787-91 2003 |
| PubMed ID: 14561894 |
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| Vincent BJ, Myers JS, Ho HJ, Kilroy GE, Walker JA, Watkins WS, Jorde LB, Batzer MA, Following the LINEs: an analysis of primate genomic variation at human-specific LINE-1 insertion sites. Mol Biol Evol20(8):1338-48 2003 |
| PubMed ID: 12777507 |
| PDL at Senescence |
104 |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
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