Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Family Member 10
Relation to Proband niece
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The donor is 50% at risk for Alzheimer's disease. Donor's paternal aunt, deceased father, and deceased paternal grandfather were affected. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 8/14/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 4. A lymphoblast culture from same donor is AG08374A. Donor's brother is AG08376 Lymphoblast and her mother is AG08381.
PDL at Freeze 4
Passage Frozen 2
 
Remark The donor is 50% at risk for Alzheimer's disease. Donor's paternal aunt, deceased father, and deceased paternal grandfather were affected. The skin biopsy was taken ante-mortem from the forearm. Culture was initiated on 8/14/85 using explants of minced skin. The cell morphology is fibroblast-like. Culture was frozen at PDL 4. A lymphoblast culture from same donor is AG08374A. Donor's brother is AG08376 Lymphoblast and her mother is AG08381.
No data is available
No data is available
Cumulative PDL at Freeze 4
Passage Frozen 2
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 15% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA