AG08346
Fibroblast from Skin, Arm
Description:
MACACA NEMESTRINA
ORDER: PRIMATES
|
Repository
|
NIA Aging Cell Culture Repository
|
|
Permit Requirements
|
CITES PERMIT REQUIRED IF SHIPPING OUTSIDE OF USA
|
| Subcollection |
Nonhuman Primates
Animals |
|
Biopsy Source
|
Arm
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
42,XY
|
|
Species
|
Macaca nemestrina
|
|
Common Name
|
pigtailed macaque
|
|
Remarks
|
|
| PDL at Senescence |
34 |
| PDL at Freeze |
2 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Genbank |
COX1 |
| Remark |
The skin biopsy was taken post-mortem on 7/25/85 from the inner aspect of the upper arm of a fetus of 130 days gestational age. The culture was initiated using explants of minced skin tissue. Cell morphology is fibroblast-like. The karyotype is 42,XY with 8% of the cells examined showing random chromosome loss. Other cultures from this monkey are AG08347 and AG08351. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
34 |
| Cumulative PDL at Freeze |
2 |
| Passage Frozen |
1 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
|
|