Repository NIA Aging Cell Culture Repository
Subcollection Animals
Biopsy Source Eye
Cell Type Epithelial
Tissue Type Lens
Transformant Untransformed
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 44,XX
Species Oryctolagus cuniculus
Common Name rabbit
Remarks The culture was initiated on 9/2/79 from the ocular lens capsule of a New Zealand White rabbit. Cell morphology is epithelial-like. The karyotype is 44,XX; normal diploid female.
PDL at Freeze 112
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis
 
Remark The culture was initiated on 9/2/79 from the ocular lens capsule of a New Zealand White rabbit. Cell morphology is epithelial-like. The karyotype is 44,XX; normal diploid female.
Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999
PubMed ID: 10559316
 
Megraud F, Gamon LB, McGarrity GJ, Characterization of Spiroplasma mirum (suckling mouse cataract agent) in a rabbit lens cell culture. Infect Immun42:1168-75 1983
PubMed ID: 6642663
 
Reddan JR, Friedman TB, Mostafapour MK, Bondy RL, Sutherland SH, McGee SJ, Goldenberg EM, Donor age influences the growth of rabbit lens epithelial cells in vitro. Vision Res21:11-23 1981
PubMed ID: 7269278
No data is available
Cumulative PDL at Freeze 112
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA