Repository NIGMS Human Genetic Cell Repository
Subcollection Old Order Amish Major Affective Disorder
Heritable Diseases
Class Disorders of the Nervous System
Quantity 50 µg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Caucasian
Ethnicity AMISH
Family Member 19
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Clinically affected; type I bipolar disorder (BPI); onset at age 29; hospitalized five times for depressive and manic-depressive illness; psychotic; treated and remained on medication for 20 years with only mini-episodes until a MDD and hospitalization at age 59; somatic concerns prominent
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark Clinically affected; type I bipolar disorder (BPI); onset at age 29; hospitalized five times for depressive and manic-depressive illness; psychotic; treated and remained on medication for 20 years with only mini-episodes until a MDD and hospitalization at age 59; somatic concerns prominent
Huff MO, Li XP, Ginns E, El-Mallakh RS, Effect of ethacrynic acid on the sodium- and potassium-activated adenosine triphosphatase activity and expression in Old Order Amish bipolar individuals Journal of affective disorders123:303-7 2009
PubMed ID: 19880188
View pedigree 
Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium